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Extracellular vesicles microRNA analysis in type 1 autoimmune pancreatitis: Increased expression of microRNA-21.
https://kmu.repo.nii.ac.jp/records/76
https://kmu.repo.nii.ac.jp/records/76e2447286-34be-4aad-8bd4-631a3b8de9f9
| 名前 / ファイル | ライセンス | アクション |
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論文要旨（要約） (159.8 kB)
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審査結果要旨 (95.0 kB)
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主論文全文 (265.3 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-18 | |||||
| タイトル | ||||||
| タイトル | Extracellular vesicles microRNA analysis in type 1 autoimmune pancreatitis: Increased expression of microRNA-21. | |||||
| タイトル | ||||||
| タイトル | Extracellular vesicles microRNA analysis in type 1 autoimmune pancreatitis: Increased expression of microRNA-21. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 別タイトル | ||||||
| その他のタイトル | （１型自己免疫性膵炎 では 細胞外小胞由来 microRNA 21 が高発現している ） | |||||
| 著者 |
中丸, 洸
× 中丸, 洸× Nakamaru, Koh× Tomiyama, Takashi× Kobayashi, Sanshiro× Ikemune, Manami× Tsukuda, Satoshi× Ito, Takashi× Tanaka, Toshihiro× Yamaguchi, Takashi× Ando, Yugo× Ikeura, Tsukasa× Fukui, Toshiro× Nishio, Akiyoshi× Takaoka, Makoto× Uchida, Kazushige× Leung, Patrick S C× Gershwin, M E× Okazaki, Kazuichi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The molecular basis of type 1 autoimmune pancreatitis (AIP) remains unclear. Recent attention on the role of extracellular vesicles microRNA (EV miRNA) in immune homeostasis has prompted us to perform an extensive miRNA screening of serum-derived EV in AIP. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | EV miRNA expression was analyzed using microarrays in AIP, chronic pancreatitis (CP), and healthy adult (HC) samples (n = 10 from each group). Differences in signals, > 3 or <1/3 times, represented significant differences in expression. Another cohort of AIP (n = 14), CP (n = 10), and HC (n = 10) samples of EV miRNA was analyzed using reverse-transcription polymerase chain reaction (RT-PCR). miRNA expression in pancreatic tissues was evaluated using in situ hybridization (ISH) in three additional subjects from each group. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Signals of eight miRNAs (miR-659-3p, -27a-3p, -99a-5p, -21-5p, -205-5p, -100-5p, -29c-3p, and -125b-1-3p) were significantly higher, while those of two miRNAs (miR-4252 and -5004-5p) were significantly lower in AIP than in HC. EV miR-21-5p was significantly up-regulated in AIP than in HC (P = 0.035) and CP (P = 0.048). The number of miR-21-5p positive inflammatory cells was significantly elevated in AIP than in CP (P = 0.014). | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Circulating EVs exhibited altered miRNA expression patterns with elevated miR-21-5p in AIP when compared with those in HC and CP. miR-21-5p was highly expressed in pancreatic inflammatory cells in AIP. Our data suggests that miR-21-5p may be involved in the regulation of effector pathways in the pathophysiology of AIP, thus differentiating AIP from CP. | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 関西医科大学 | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 令和元年度 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2020-03-24 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 課博第1063号 | |||||
| 書誌情報 |
Pancreatology en : Pancreatology 巻 20, 号 3, p. 318-324, 発行日 2020-04 |
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| DOI | ||||||
| 表示名 | 10.1016/j.pan.2020.02.012 | |||||
| URL | https://doi.org/10.1016/j.pan.2020.02.012 | |||||
