| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-08-18 |
| タイトル |
|
|
タイトル |
Extracellular vesicles microRNA analysis in type 1 autoimmune pancreatitis: Increased expression of microRNA-21. |
| タイトル |
|
|
タイトル |
Extracellular vesicles microRNA analysis in type 1 autoimmune pancreatitis: Increased expression of microRNA-21. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 別タイトル |
|
|
その他のタイトル |
（１型自己免疫性膵炎 では 細胞外小胞由来 microRNA 21 が高発現している ） |
| 著者 |
中丸, 洸
Nakamaru, Koh
Tomiyama, Takashi
Kobayashi, Sanshiro
Ikemune, Manami
Tsukuda, Satoshi
Ito, Takashi
Tanaka, Toshihiro
Yamaguchi, Takashi
Ando, Yugo
Ikeura, Tsukasa
Fukui, Toshiro
Nishio, Akiyoshi
Takaoka, Makoto
Uchida, Kazushige
Leung, Patrick S C
Gershwin, M E
Okazaki, Kazuichi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The molecular basis of type 1 autoimmune pancreatitis (AIP) remains unclear. Recent attention on the role of extracellular vesicles microRNA (EV miRNA) in immune homeostasis has prompted us to perform an extensive miRNA screening of serum-derived EV in AIP. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
EV miRNA expression was analyzed using microarrays in AIP, chronic pancreatitis (CP), and healthy adult (HC) samples (n = 10 from each group). Differences in signals, > 3 or <1/3 times, represented significant differences in expression. Another cohort of AIP (n = 14), CP (n = 10), and HC (n = 10) samples of EV miRNA was analyzed using reverse-transcription polymerase chain reaction (RT-PCR). miRNA expression in pancreatic tissues was evaluated using in situ hybridization (ISH) in three additional subjects from each group. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Signals of eight miRNAs (miR-659-3p, -27a-3p, -99a-5p, -21-5p, -205-5p, -100-5p, -29c-3p, and -125b-1-3p) were significantly higher, while those of two miRNAs (miR-4252 and -5004-5p) were significantly lower in AIP than in HC. EV miR-21-5p was significantly up-regulated in AIP than in HC (P = 0.035) and CP (P = 0.048). The number of miR-21-5p positive inflammatory cells was significantly elevated in AIP than in CP (P = 0.014). |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Circulating EVs exhibited altered miRNA expression patterns with elevated miR-21-5p in AIP when compared with those in HC and CP. miR-21-5p was highly expressed in pancreatic inflammatory cells in AIP. Our data suggests that miR-21-5p may be involved in the regulation of effector pathways in the pathophysiology of AIP, thus differentiating AIP from CP. |
| 学位名 |
|
|
学位名 |
博士（医学） |
| 学位授与機関 |
|
|
|
学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
令和元年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2020-03-24 |
| 学位授与番号 |
|
|
学位授与番号 |
課博第1063号 |
| 書誌情報 |
Pancreatology
en : Pancreatology
巻 20,
号 3,
p. 318-324,
発行日 2020-04
|
| DOI |
|
|
|
10.1016/j.pan.2020.02.012 |
|
|
https://doi.org/10.1016/j.pan.2020.02.012 |
論文要旨（要約） (159.8 kB)
