| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-08-26 |
| タイトル |
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タイトル |
TGF-β Signaling Accelerates Senescence of Human Bone-Derived CD271 and SSEA-4 Double-Positive Mesenchymal Stromal Cells |
| タイトル |
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タイトル |
TGF-β Signaling Accelerates Senescence of Human Bone-Derived CD271 and SSEA-4 Double-Positive Mesenchymal Stromal Cells |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
| 別タイトル |
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その他のタイトル |
TGF-β シグナルはヒト骨組織由来 CD271、SSEA4 共陽性間葉系幹細胞の 細胞老化を促進する |
| 著者 |
河村, 孟
Kawamura, Hiroshi
Nakatsuka, Ryusuke
Matsuoka, Yoshikazu
Sumide, Keisuke
Fujioka, Tatsuya
Asano, Hiroaki
Iida, Hirokazu
Sonoda, Yoshiaki
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
It is generally thought that the proliferative capacity and differentiation potential of somatic stem cells, including mesenchymal stromal/stem cells (MSCs) and hematopoietic stem cells, decline with age. We investigated the effects of aging on human bone-derived MSCs expressing CD271 and SSEA-4 (double-positive MSCs [DPMSCs]). The percentages of DPMSCs in bone tissue decreased significantly with age. The DPMSCs from elderly patients (old DPMSCs) showed cellular senescence, which was evidenced by low growth potential, high senescence-associated β-galactosidase activity, and elevated p16 and p21 CDK inhibitor levels. Moreover, old DPMSCs showed weak osteogenic differentiation potential and less hematopoiesis-supporting activity in comparison with young DPMSCs. Interestingly, the addition of transforming growth factor β2 (TGF-β2) induced cellular senescence in young DPMSCs. With the exception of the adipogenic differentiation potential, all of the aging phenomena observed in old DPMSCs were reversed by the addition of anti-TGF-β antibodies. These results suggest that, in part, old DPMSCs accelerate cellular senescence through TGF-β signaling. |
| 学位名 |
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学位名 |
博士(医学) |
| 学位授与機関 |
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学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
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内容記述タイプ |
Other |
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内容記述 |
平成29年度 |
| 学位授与年月日 |
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学位授与年月日 |
2018-03-27 |
| 学位授与番号 |
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学位授与番号 |
課博第1020号 |
| 書誌情報 |
Stem Cell Reports
en : Stem Cell Reports
巻 10,
号 3,
p. 920-932,
発行日 2018
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| DOI |
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10.1016/j.stemcr.2018.01.030 |
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https://doi.org/10.1016/j.stemcr.2018.01.030 |
論文要旨（要約） (258.0 kB)
