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TGF-β Signaling Accelerates Senescence of Human Bone-Derived CD271 and SSEA-4 Double-Positive Mesenchymal Stromal Cells
https://kmu.repo.nii.ac.jp/records/142
https://kmu.repo.nii.ac.jp/records/1424d635f04-ca3b-44af-8e12-f3a71bb316a6
| 名前 / ファイル | ライセンス | アクション |
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論文要旨（要約） (258.0 kB)
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審査結果要旨 (73.0 kB)
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主論文 (10.8 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2020-08-26 | |||||
| タイトル | ||||||
| タイトル | TGF-β Signaling Accelerates Senescence of Human Bone-Derived CD271 and SSEA-4 Double-Positive Mesenchymal Stromal Cells | |||||
| タイトル | ||||||
| タイトル | TGF-β Signaling Accelerates Senescence of Human Bone-Derived CD271 and SSEA-4 Double-Positive Mesenchymal Stromal Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 別タイトル | ||||||
| その他のタイトル | TGF-β シグナルはヒト骨組織由来 CD271、SSEA4 共陽性間葉系幹細胞の 細胞老化を促進する | |||||
| 著者 |
河村, 孟
× 河村, 孟× Kawamura, Hiroshi× Nakatsuka, Ryusuke× Matsuoka, Yoshikazu× Sumide, Keisuke× Fujioka, Tatsuya× Asano, Hiroaki× Iida, Hirokazu× Sonoda, Yoshiaki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It is generally thought that the proliferative capacity and differentiation potential of somatic stem cells, including mesenchymal stromal/stem cells (MSCs) and hematopoietic stem cells, decline with age. We investigated the effects of aging on human bone-derived MSCs expressing CD271 and SSEA-4 (double-positive MSCs [DPMSCs]). The percentages of DPMSCs in bone tissue decreased significantly with age. The DPMSCs from elderly patients (old DPMSCs) showed cellular senescence, which was evidenced by low growth potential, high senescence-associated β-galactosidase activity, and elevated p16 and p21 CDK inhibitor levels. Moreover, old DPMSCs showed weak osteogenic differentiation potential and less hematopoiesis-supporting activity in comparison with young DPMSCs. Interestingly, the addition of transforming growth factor β2 (TGF-β2) induced cellular senescence in young DPMSCs. With the exception of the adipogenic differentiation potential, all of the aging phenomena observed in old DPMSCs were reversed by the addition of anti-TGF-β antibodies. These results suggest that, in part, old DPMSCs accelerate cellular senescence through TGF-β signaling. | |||||
| 学位名 | ||||||
| 学位名 | 博士(医学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 関西医科大学 | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 平成29年度 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2018-03-27 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 課博第1020号 | |||||
| 書誌情報 |
Stem Cell Reports en : Stem Cell Reports 巻 10, 号 3, p. 920-932, 発行日 2018 |
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| DOI | ||||||
| 表示名 | 10.1016/j.stemcr.2018.01.030 | |||||
| URL | https://doi.org/10.1016/j.stemcr.2018.01.030 | |||||
