| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-11-13 |
| タイトル |
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|
タイトル |
Thyroid Hormone Facilitates in vitro Decidualization of Human Endometrial Stromal Cells via Thyroid Hormone Receptors. |
| タイトル |
|
|
タイトル |
Thyroid Hormone Facilitates in vitro Decidualization of Human Endometrial Stromal Cells via Thyroid Hormone Receptors. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 別タイトル |
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その他のタイトル |
( 甲状腺ホルモンは甲状腺ホルモン受容体を介してヒト子宮内膜間質細胞の 脱落膜化を誘導する) |
| 著者 |
小林, 真以子
Kakita-Kobayashi, Maiko
Murata, Hiromi
Nishigaki, Akemi
Hashimoto, Yoshiko
Komiya, Shinnosuke
Tsubokura, Hiroaki
Kido, Takeharu
Kida, Naoko
Tsuzuki-Nakao, Tomoko
Matsuo, Yoshiyuki
Bono, Hidemasa
Hirota, Kiichi
Okada, Hidetaka
|
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Endometrial stromal cells differentiate into decidual cells through the process of decidualization. This differentiation is critical for embryo implantation and the successful establishment of pregnancy. Recent epidemiological studies have suggested that thyroid hormone is important in the endometrium during implantation, and it is commonly believed that thyroid hormone is essential for proper development, differentiation, growth, and metabolism. This study aimed to investigate the impact of thyroid hormone on decidualization in human endometrial stromal cells (hESCs) and define its physiological roles in vitro by gene targeting. To identify the expression patterns of thyroid hormone, we performed gene expression profiling of hESCs during decidualization after treating them with the thyroid hormone levothyroxine (LT4). A major increase in decidual response was observed after combined treatment with ovarian steroid hormones and thyroid hormone. Moreover, LT4 treatment also affected the regulation of many transcription factors important for decidualization. We found that type 3 deiodinase, which is particularly important in fetal and placental tissues, was upregulated during decidualization in the presence of thyroid hormone. Further, it was observed that progesterone receptor, an ovarian steroid hormone receptor, was involved in thyroid hormone-induced decidualization. In the absence of thyroid hormone receptor (TR), due to the simultaneous silencing of TRα and TRβ, thyroid hormone expression was unchanged during decidualization. In summary, we demonstrated that thyroid hormone is essential for decidualization in the endometrium. This is the first in vitro study to find impaired decidualization as a possible cause of infertility in subclinical hypothyroidism (SCH) patients. |
| 学位名 |
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|
学位名 |
博士（医学） |
| 学位授与機関 |
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|
学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
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内容記述タイプ |
Other |
|
内容記述 |
令和2年度 |
| 学位授与年月日 |
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学位授与年月日 |
2020-09-29 |
| 学位授与番号 |
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学位授与番号 |
課博第1071号 |
| 書誌情報 |
Endocrinology
en : Endocrinology
巻 161,
号 6,
発行日 2020-06-01
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| DOI |
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|
|
10.1210/endocr/bqaa049 |
|
|
https://doi.org/10.1210/endocr/bqaa049 |
論文要旨（要約） (229.4 kB)
