| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-08-19 |
| タイトル |
|
|
タイトル |
Shared expression of mucin12 in Ascaris lumbricoides and the human small intestine. |
| タイトル |
|
|
タイトル |
Shared expression of mucin12 in Ascaris lumbricoides and the human small intestine. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Animals |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ascariasis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ascaris lumbricoides |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Helminth Proteins |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Humans |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Intestine, Small |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mucins |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Protein Transport |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 別タイトル |
|
|
その他のタイトル |
( ヒト回虫 とヒト小腸粘膜に共通に局在する mucin12） |
| 著者 |
林, 猪都子
Hayashi, Itoko
Kanda, Seiji
Lamaningao, Pheophet
Mishima, Nobuyuki
Nishiyama, Toshimasa
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
This study focuses on the host-parasite relationship of human Ascaris lumbricoides, which is a parasite of the small intestine and is also one of the commonest parasites worldwide. As part of this investigation, we examined the host-parasite relationship assuming that there is a common antigenicity, shared protein between A. lumbricoides and human small intestinal mucosa, using molecular techniques. We obtained three DNA clones from human colon cDNA library by screening for anti-A. lumbricoides polyclonal antibodies. The transmembrane mucin12 gene was identified after sequencing analysis of these clones. Specific signals of immunostaining with polyclonal anti-mucin12 antibodies were observed in the mucous secretory organs, epidermis, and intestinal canal of A. lumbricoides. These signals disappeared when immunohistochemistry was performed using pre-absorbed polyclonal antibodies with a specific peptide. These results suggest that mucin12 is localized in the mucous secretory organs in the epidermis of A. lumbricoides. Furthermore, we examined the site of mucin12 localization in the host; specific mucin12 signals were observed on the mucosal epithelia present around intestinal crypts and villi of the small intestine. Therefore, we suggest that mucin12 is a protein that shows common antigenicity in both A. lumbricoides and its host. It is presumed that adult A. lumbricoides live in their preferred environment, which is the small intestine, by secreting mucin12 to avoid being attacked by the host immune system. |
| 学位名 |
|
|
学位名 |
博士（医学） |
| 学位授与機関 |
|
|
|
学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
令和元年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2019-09-24 |
| 学位授与番号 |
|
|
学位授与番号 |
論博第731号 |
| 書誌情報 |
Molecular and biochemical parasitology
en : Molecular and biochemical parasitology
巻 227,
p. 19-24,
発行日 2019
|
| DOI |
|
|
|
10.1016/j.molbiopara.2018.11.004 |
|
|
https://doi.org/10.1016/j.molbiopara.2018.11.004 |
論文要旨（要約） (179.2 kB)
