| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-07-20 |
| タイトル |
|
|
タイトル |
Simple and efficient method for consecutive inactivation-cryopreservation of porcine skin grafts. |
| タイトル |
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|
タイトル |
Simple and efficient method for consecutive inactivation-cryopreservation of porcine skin grafts. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 別タイトル |
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その他のタイトル |
簡便で効率的なブタ皮膚移植片の不活化―凍結保存法 |
| 著者 |
益岡, 弘
Masuoka, Hiromu
Morimoto, Naoki
Mahara, Atsushi
Sakamoto, Michiharu
Mitsui, Toshihito
Kakudo, Natsuko
Kusumoto, Kenji
Yamaoka, Tetsuji
|
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
We previously reported that inactivation treatment by high hydrostatic pressurization (HHP) has potential utility as a novel skin regeneration therapy for various skin tumors. In this study, we evaluated whether glycerol-cryopreservation could be applied in order to preserve inactivated skin by HHP using a porcine model. Twenty full-thickness skin grafts (1.5 × 1.5 cm) were prepared from a minipig. The skin samples were inactivated by the HHP in normal saline or glycerol/fructose solution, followed by cryopreservation for 5 weeks at - 80 °C in each same solution. Another 10 grafts immediately after inactivation were prepared as non-cryopreserved controls. Nine grafts in each group were randomly implanted on the fascia of a host pig and removed at 1, 4 and 11 weeks after grafting. All grafts showed engraftment macroscopically. Hematoxylin eosin staining showed the cellular components in all areas of the dermis at 4 and 11 weeks after grafting, and immunohistochemical staining for CD31 showed the presence of capillaries in the grafts in all groups. The surface and cross-sectional areas of grafts in the normal saline solution cryopreserved group decreased between 1 and 11 weeks, whereas these areas in the glycerol cryopreserved group did not decrease significantly. Glycerol cryopreservation may therefore be a simple and efficient method for preserving porcine skin inactivated by HHP. |
| 学位名 |
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学位名 |
博士（医学） |
| 学位授与機関 |
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学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
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内容記述タイプ |
Other |
|
内容記述 |
令和元年度 |
| 学位授与年月日 |
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|
学位授与年月日 |
2020-03-24 |
| 学位授与番号 |
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|
学位授与番号 |
論博第737号 |
| 書誌情報 |
Journal of artificial organs
en : Journal of artificial organs
巻 23,
号 2,
p. 147-155,
発行日 2020-06
|
| DOI |
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10.1007/s10047-019-01142-2 |
|
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https://doi.org/10.1007/s10047-019-01142-2 |
論文要旨（要約） (291.1 kB)
