| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-11-25 |
| タイトル |
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|
タイトル |
Specific Smad2/3 Linker Phosphorylation Indicates Esophageal Non-neoplastic and Neoplastic Stem-Like Cells and Neoplastic Development. |
| タイトル |
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|
タイトル |
Specific Smad2/3 Linker Phosphorylation Indicates Esophageal Non-neoplastic and Neoplastic Stem-Like Cells and Neoplastic Development. |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
| 別タイトル |
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その他のタイトル |
( リンカー部スレオニンリン酸化 Smad2/3 蛋白 発現による 食道上皮幹細胞 、 腫瘍幹細胞の同定と 食道腫瘍発生進展メカニズムの 解析） |
| 著者 |
堀谷, 俊介
Horitani, Shunsuke
Fukui, Toshiro
Tanimura, Yuji
Matsumoto, Yasushi
Miyamoto, Sachi
Tanaka, Toshihiro
Tomiyama, Takashi
Ikeura, Tsukasa
Ando, Yugo
Nishio, Akiyoshi
Okazaki, Kazuichi
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
There is little known about stem cells in human non-neoplastic and neoplastic esophageal epithelia. We have demonstrated expression of linker threonine-phosphorylated Smad2/3 (pSmad2/3L-Thr), suggesting presence of stem-like cells in mouse esophageal epithelium, and identified presence of pSmad2/3L-Thr-positive cells that might function as cancer stem cells in mouse model of colorectal carcinoma. |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We explore whether pSmad2/3L-Thr can be used as a biomarker for stem cells of human esophageal epithelia and/or neoplasms. |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We have used esophageal tissues from inpatients undergoing endoscopic submucosal dissection and performed double immunofluorescent staining of pSmad2/3L-Thr and Ki67, CDK4, p63, Sox2, CK14, p53, ALDH1, CD44 or D2-40 after which the sections were stained with hematoxylin and eosin. |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
pSmad2/3L-Thr-positive cells showed immunohistochemical co-localization with CDK4, p63, CD44 and Sox2 in the basal and parabasal layers of non-neoplastic esophageal epithelia. In esophageal neoplasms, they showed immunohistochemical co-localization with p53, CDK4, ALDH1 and CD44. There was a significant increase in the percentage of pSmad2/3L-Thr-positive cells in the p53-positive neoplastic cell population with development of esophageal neoplasia. pSmad2/3L-Thr-positive cells localized to the lower section of low-grade intraepithelial neoplasia and were observed up to the upper section in carcinoma in situ. In invasive squamous cell carcinoma, they were scattered throughout the tumor with disappearance of polarity and were found in intraepithelial primary lesions and sites of submucosal and vessel invasion. |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We determined significant expression of pSmad2/3L-Thr in human esophageal non-neoplastic and neoplastic epithelia, indicating that these are epithelial stem-like cells and cancer stem cells, respectively, that correlate with developing esophageal neoplasms. |
| 学位名 |
|
|
学位名 |
博士（医学） |
| 学位授与機関 |
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学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
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内容記述タイプ |
Other |
|
内容記述 |
令和2年度 |
| 学位授与年月日 |
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学位授与年月日 |
2020-09-29 |
| 学位授与番号 |
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学位授与番号 |
課博第1072号 |
| 書誌情報 |
Digestive diseases and sciences
en : Digestive diseases and sciences
発行日 2020-07-23
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| DOI |
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|
|
10.1007/s10620-020-06489-8 |
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https://doi.org/10.1007/s10620-020-06489-8 |
論文要旨（要約） (144.2 kB)
