| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-10-29 |
| タイトル |
|
|
タイトル |
Improved viability of murine skin flaps using a gelatin hydrogel sheet impregnated with bFGF. |
| タイトル |
|
|
タイトル |
Improved viability of murine skin flaps using a gelatin hydrogel sheet impregnated with bFGF. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 別タイトル |
|
|
その他のタイトル |
（ bFGF とゼラチンハイドロゲルを用いたマウス皮弁生着域延長効果) |
| 著者 |
日原, 正勝
Hihara, Masakatsu
Kakudo, Natsuko
Morimoto, Naoki
Hara, Tomoya
Lai, Fangyuan
Jo, Junichiro
Tabata, Yasuhiko
Kusumoto, Kenji
|
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Basic fibroblast growth factor (bFGF) promotes epithelial cell proliferation and angiogenesis but its clinical applications are limited by its short half-life and low retention. Recently developed gelatin hydrogel sheets able to release physiologically active substances in a controlled manner have the potential to overcome these issues. In this study, the effects of gelatin hydrogel sheets impregnated with bFGF on flap survival and angiogenesis were examined in a murine skin flap model. A flap of 1 × 3 cm was generated on the backs of 60 C57BL/6 mice. The mice were divided into five groups (n = 12/group): Group I, untreated; Group II, treated with a gelatin hydrogel sheet impregnated with saline; Group III, treated with bFGF (50 µg) without sheets; Groups IV and V, treated with gelatin hydrogel sheets impregnated with 50 and 100 µg of bFGF, respectively. On the seventh day after surgery, the flap survival area and vascular network were examined and hematoxylin and eosin and von Willebrand factor staining were used for histological examinations. The flap survival areas were significantly larger in Groups IV and V than in other groups. The area of new vessels was significantly larger in Group IV than in the other groups. In the murine skin flap model, gelatin hydrogel sheets impregnated with bFGF promoted angiogenesis and improved flap survival. These findings support the use of bFGF-impregnated gelatin hydrogel sheets for improving ischemic flap survival in clinical settings. |
| 学位名 |
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|
学位名 |
博士（医学） |
| 学位授与機関 |
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学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
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内容記述タイプ |
Other |
|
内容記述 |
令和2年度 |
| 学位授与年月日 |
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|
学位授与年月日 |
2020-09-29 |
| 学位授与番号 |
|
|
学位授与番号 |
課博第741号 |
| 書誌情報 |
Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs
en : Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs
発行日 2020-07-06
|
| DOI |
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10.1007/s10047-020-01188-7 |
|
|
https://doi.org/10.1007/s10047-020-01188-7 |
論文要旨（要約） (144.5 kB)
