| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-08-26 |
| タイトル |
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|
タイトル |
Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells |
| タイトル |
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|
タイトル |
Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 別タイトル |
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その他のタイトル |
モルモット膵臓導管細胞管腔側膜における陰イオン交換輸送体の 電気生理学的性質 |
| 著者 |
Andharia, Naaz
Andharia, N.
Hayashi, M.
Matsuda, H.
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The pancreatic duct epithelium secretes the HCO3--rich pancreatic juice. The HCO3- transport across the luminal membrane has been proposed to be mediated by SLC26A Cl--HCO3- exchangers. To examine the electrophysiological properties of Cl--HCO3- exchangers, we directly measured HCO3- conductance in the luminal membrane of the interlobular pancreatic duct cells from guinea pigs using an inside-out patch-clamp technique. Intracellular HCO3- increased the HCO3- conductance with a half-maximal effective concentration value of approximately 30 mM. The selectivity sequence based on permeability ratios was SCN- (1.4) > Cl- (1.2) = gluconate (1.1) = I- (1.1) = HCO3- (1.0) > methanesulfonate (0.6). The sequence of the relative conductance was HCO3- (1.0) > SCN- (0.7) = I- (0.7) > Cl- (0.5) = gluconate (0.4) > methanesulfonate (0.2). The current dependent on intracellular HCO3- was reduced by replacement of extracellular Cl- with gluconate or by H2DIDS, an inhibitor of Cl--HCO3- exchangers. RT-PCR analysis revealed that the interlobular and main ducts expressed all SLC26A family members except Slc26a5 and Slc26a8. SLC26A1, SLC26A4, SLC26A6, and SLC26A10 were found to be localized to the luminal membrane of the guinea pig pancreatic duct by immunohistochemistry. These results demonstrate that these SLC26A Cl--HCO3- exchangers may mediate the electrogenic HCO3- transport through the luminal membrane and may be involved in pancreatic secretion in guinea pig ducts. |
| 学位名 |
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学位名 |
博士(医学) |
| 学位授与機関 |
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学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
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内容記述タイプ |
Other |
|
内容記述 |
平成29年度 |
| 学位授与年月日 |
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学位授与年月日 |
2018-03-27 |
| 学位授与番号 |
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学位授与番号 |
課博第1019号 |
| 書誌情報 |
Pflügers Archiv - European Journal of Physiology
en : Pflügers Archiv - European Journal of Physiology
巻 470,
号 6,
p. 897-907,
発行日 2018
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| DOI |
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10.1007/s00424-018-2116-1 |
|
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https://doi.org/10.1007/s00424-018-2116-1 |
論文要旨（要約） (186.3 kB)
