| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-08-26 |
| タイトル |
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|
タイトル |
Near-infrared fluorescence imaging and photodynamic therapy with indocyanine green lactosome has antineoplastic effects for hepatocellular carcinoma |
| タイトル |
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タイトル |
Near-infrared fluorescence imaging and photodynamic therapy with indocyanine green lactosome has antineoplastic effects for hepatocellular carcinoma |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
| 別タイトル |
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その他のタイトル |
インドシアニングリーンラクトソームを用いた近赤外蛍光イメージング及び 光線力学療法の肝細胞癌に対する抗腫瘍効果 |
| 著者 |
津田, 匠
Tsuda, Takumi
Kaibori, Masaki
Hishikawa, Hidehiko
Nakatake, Richi
Okumura, Tadayoshi
Ozeki, Eiichi
Hara, Isao
Morimoto, Yuji
Yoshii, Kengo
Kon, Masanori
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Background
Anticancer agents and operating procedures have been developed for hepatocellular carcinoma (HCC) patients, but their prognosis remains poor. It is necessary to develop novel diagnostic and therapeutic strategies for HCC to improve its prognosis. Lactosome is a core-shell-type polymeric micelle, and enclosing labeling or anticancer agents into this micelle enables drug delivery. In this study, we investigated the diagnostic and therapeutic efficacies of indocyanine green (ICG)-loaded lactosome for near-infrared fluorescence (NIF) imaging and photodynamic therapy (PDT) for HCC.
Methods
The human HCC cell line HuH-7 was treated with ICG or ICG-lactosome, followed by PDT, and the cell viabilities were measured (in vitro PDT efficiency). For NIF imaging, HuH-7 cells were subcutaneously transplanted into BALB/c nude mice, followed by intravenous administration of ICG or ICG-lactosome. The transplanted animals were treated with PDT, and the antineoplastic effects were analyzed (in vivo PDT efficiency).
Results
PDT had toxic effects on HuH-7 cells treated with ICG-lactosome, but not ICG alone. NIF imaging revealed that the fluorescence of tumor areas in ICG-lactosome-treated animals was higher than that of contralateral regions at 24 h after injection and thereafter. PDT exerted immediate and continuous phototoxic effects in the transplanted mice treated with ICG-lactosome.
Conclusions
Our results demonstrate that ICG-lactosome accumulated in xenograft tumors, and that PDT had antineoplastic effects on these malignant implants. NIF imaging and PDT with ICG-lactosome could be useful diagnostic and/or therapeutic strategies for HCC. |
| 学位名 |
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学位名 |
博士(医学) |
| 学位授与機関 |
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学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
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内容記述タイプ |
Other |
|
内容記述 |
平成29年度 |
| 学位授与年月日 |
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学位授与年月日 |
2018-03-27 |
| 学位授与番号 |
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学位授与番号 |
課博第1016号 |
| 書誌情報 |
PLOS ONE
en : PLOS ONE
巻 12,
号 8,
p. e0183527,
発行日 2017
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| DOI |
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10.1371/journal.pone.0183527 |
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https://doi.org/10.1371/journal.pone.0183527 |
論文要旨（要約） (135.4 kB)
