| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-08-26 |
| タイトル |
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タイトル |
Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis |
| タイトル |
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タイトル |
Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
| 著者 |
柳川, 雅人
Yanagawa, Masato
Uchida, Kazushige
Ando, Yugo
Tomiyama, Takashi
Yamaguchi, Takashi
Ikeura, Tsukasa
Fukui, Toshiro
Nishio, Akiyoshi
Uemura, Yoshiko
Miyara, Takayuki
Okamoto, Hiroyuki
Satoi, Souhei
Okazaki, Kazuichi
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Background: Pathophysiology of type 1 autoimmune pancreatitis (AIP) is still unclear. We previously reported that M2 macrophages might play an important role in type 1 AIP. Recently, it has been reported that basophils regulate differentiation to M2 macrophages. In this study, we investigated basophils from the pancreatic tissue and peripheral blood of individuals with type 1 AIP.
Methods: By using immunohistochemistry, we investigated basophils in pancreatic tissue from 13 patients with type 1 AIP and examined expression of toll-like receptors (TLRs) by these cells. Additionally, we obtained peripheral blood samples from 27 healthy subjects, 40 patients with type 1 AIP, 8 patients with alcoholic chronic pancreatitis, 10 patients with bronchial asthma, and 10 patients with atopic dermatitis, and analyzed activation of basophils by stimulating them with ligands of TLR1-9. We also compared TLR expression in basophils from the tissue and blood samples.
Results: Basophils were detected in pancreatic tissues from 10 of 13 patients with type 1 AIP. Flow cytometric analysis revealed that the ratios of basophils activated by TLR4 stimulation in type 1 AIP (9.875 ± 1.148%) and atopic dermatitis (11.768 ± 1.899%) were significantly higher than those in healthy subjects (5.051 ± 0.730%; P < 0.05). Levels of basophils activated by TLR2 stimulation were higher in seven type 1 AIP cases. Furthermore, stimulation of TLR2 and/or TLR4, which were expressed by basophils in pancreas, activated basophils in peripheral blood.
Conclusions: Basophils activated via TLR signaling may play an important role in the pathophysiology of type 1 AIP. |
| 学位名 |
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学位名 |
博士(医学) |
| 学位授与機関 |
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学位授与機関名 |
関西医科大学 |
| 学位授与年度 |
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内容記述タイプ |
Other |
|
内容記述 |
平成29年度 |
| 学位授与年月日 |
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学位授与年月日 |
2018-03-27 |
| 学位授与番号 |
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学位授与番号 |
課博第1014号 |
| 書誌情報 |
Journal of Gastroenterology
en : Journal of Gastroenterology
巻 53,
号 3,
p. 449-460,
発行日 2017
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| DOI |
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10.1007/s00535-017-1390-6 |
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https://doi.org/10.1007/s00535-017-1390-6 |
論文要旨（要約） (138.0 kB)
